N-ethylmaleimide-sensitive mutant (beta Val-153-->Cys) Escherichia coli F1-ATPase: cross-linking of the mutant beta subunit with the alpha subunit.

doi:10.1016/0014-5793(94)00963-5

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Article

N-ethylmaleimide-sensitive mutant (beta Val-153-->Cys) Escherichia coli F1-ATPase: cross-linking of the mutant beta subunit with the alpha subunit.

FEBS letters - 26 Sept 1994

Iwamoto A, Orita-Saita Y, Maeda M, Futai M

Abstract excerpt

A beta subunit mutation, beta Val-153-->Cys, in the glycine-rich sequence (phosphate-binding loop) of Escherichia coli F1 was constructed. Like vacuolar-type ATPase, the mutant enzyme was inhibited by N-ethylmaleimide (NEM) and labeled with [14C]NEM. The inhibition and labeling were prevented by...

Topics

Adenosine Triphosphate
Amino Acid Sequence
Cross-Linking Reagents
Cysteine
Escherichia coli
Ethylmaleimide
Molecular Sequence Data
Mutation
Proton-Translocating ATPases
Succinimides

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