Genomic DNA fingerprinting of clinical Haemophilus influenzae isolates by polymerase chain reaction amplification: comparison with major outer-membrane protein and restriction fragment length polymorphism analysis.

doi:10.1099/00222615-41-1-63

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Article

Genomic DNA fingerprinting of clinical Haemophilus influenzae isolates by polymerase chain reaction amplification: comparison with major outer-membrane protein and restriction fragment length polymorphism analysis.

Journal of medical microbiology - 1 Jul 1994

van Belkum A, Duim B, Regelink A, Möller L, Quint W, van Alphen L

Abstract excerpt

Non-capsulate strains of Haemophilus influenzae were genotyped by analysis of variable DNA segments obtained by amplification of genomic DNA with the polymerase chain reaction (PCR fingerprinting). Discrete fragments of 100-2000 bp were obtained. The reproducibility of the procedure was assessed by comparing: (i) the fingerprints of 16 colonies of a single H. influenzae strain; (ii) isolates obtained from...

Topics

Bacterial Outer Membrane Proteins
Base Sequence
Consensus Sequence
Cystic Fibrosis
DNA Fingerprinting
DNA Primers
DNA, Bacterial
Genotype
Haemophilus Infections
Haemophilus influenzae

Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

Humans

Lung Diseases, Obstructive

Molecular Sequence Data

Polymerase Chain Reaction

Polymorphism, Restriction Fragment Length

Repetitive Sequences, Nucleic Acid

Reproducibility of Results

Sputum

Source

Open in PubMed

Identifiers

PMID: 7911842
DOI: 10.1099/00222615-41-1-63

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 29 Aug 2026. Prepared record publication-7911842-2026-08-29.1. Manifest publication-graph-cohort-2026-08-29.1.

Source receipt: PubMed via Helena Literature Corpus, retrieved 29 Aug 2026.

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