Article
Molecular cloning and polymerase chain reaction-sequence-specific oligonucleotide detection of the allele encoding the novel allospecificity HLA-Cw6.2 (Cw*1502) in Spanish gypsies.
Human immunology - 1 Aug 1993
Vilches C, de Pablo R, Herrero M J, Moreno M E, Kreisler M
Abstract excerpt
A proposed novel allospecificity, HLA-Cw6.2, has been reported to be commonly found (approximately 25%) in Spanish Gypsies. Full-length cDNAs of the allele (Cw*1502) coding for this antigen have been cloned in this study. A simple PCR-SSO method for its detection has been standardized and a corre...
Topics
- Alleles
- Amino Acid Sequence
- Base Sequence
- Cloning, Molecular
- Consensus Sequence
- DNA
- HLA-C Antigens
- Histocompatibility Testing
- Humans
- Immunophenotyping
- Molecular Sequence Data
- Oligonucleotides
- Polymerase Chain Reaction
- Polymorphism, Restriction Fragment Length
- Protein Structure, Tertiary
- Roma
- Spain
