Article
Rapid Mycobacterium species assignment and unambiguous identification of mutations associated with antimicrobial resistance in Mycobacterium tuberculosis by automated DNA sequencing.
Archives of pathology & laboratory medicine - 1 Feb 1995
Kapur V, Li L L, Hamrick M R, Plikaytis B B, Shinnick T M, Telenti A, Jacobs W R, Banerjee A, Cole S, Yuen K Y
Abstract excerpt
OBJECTIVE: To develop and demonstrate the utility of automated DNA sequencing strategies for rapid and unambiguous identification of Mycobacterium species and mutations associated with antimicrobial resistance in Mycobacterium tuberculosis. DESIGN AND SPECIMENS: A 360-base pair segment of the gene (hsp65) encoding a 65-kd heat shock protein was characterized from 91 isolates assigned to 24 Mycobacterium species...
Topics
- Alleles
- Bacteriological Techniques
- Base Sequence
- Drug Resistance, Microbial
- Genes, Bacterial
- Humans
- Molecular Sequence Data
- Mutation
- Mycobacterium
- Mycobacterium tuberculosis