Article
Allele-specific quantification of Drosophila engrailed and invected transcripts.
Proceedings of the National Academy of Sciences of the United States of America - 20 Dec 1994
Goldsborough A S, Kornberg T B
Abstract excerpt
Changes in levels of transcription can be difficult to gauge in animals with lethal mutations. For example, mutations in a regulatory region of an essential gene can have secondary consequences that complicate attempts to quantify the transcripts produced by the mutant gene. We describe a method...
Topics
- Alleles
- Animals
- Base Sequence
- DNA Primers
- Drosophila Proteins
- Drosophila melanogaster
- Gene Expression Regulation
- Genes, Insect
- Genetic Linkage
- Heterozygote
- Homeodomain Proteins
- Insect Hormones
- Larva
- Molecular Sequence Data
- Polymerase Chain Reaction
- RNA, Messenger
- Transcription Factors