Article
Characterization of fus1 of Schizosaccharomyces pombe: a developmentally controlled function needed for conjugation.
Molecular and cellular biology - 1 Jul 1995
Petersen J, Weilguny D, Egel R, Nielsen O
Abstract excerpt
In Schizosaccharomyces pombe, the fus1 mutation blocks conjugation at a point after cell contact and agglutination. The cell walls separating the mating partners are not degraded, which prevents cytoplasmic fusion. In order to investigate the molecular mechanism of conjugation, we cloned the fus1 gene and found that it is capable of encoding a 1,372-amino-acid protein with no significant similarities to other...
Topics
- Amino Acid Sequence
- Base Sequence
- Blotting, Northern
- Blotting, Western
- Cloning, Molecular
- Conjugation, Genetic
- DNA Mutational Analysis
- Fluorescent Antibody Technique
- Fungal Proteins
- Gene Expression Regulation, Fungal