Article
PCR typing of field isolates of Plasmodium falciparum.
Journal of clinical microbiology - 1 Apr 1995
Contamin H, Fandeur T, Bonnefoy S, Skouri F, Ntoumi F, Mercereau-Puijalon O
Abstract excerpt
We report on an analysis of the constraints of PCR typing of field Plasmodium falciparum isolates by using a few highly polymorphic markers, MSA-1, MSA-2, TRAP, and CS. We show that the reactions are specific for the P. falciparum species. The detection threshold (minimum number of parasites required to detect a visible band by ethidium bromide) differed from one marker to the other and, within one locus, from...
Topics
- Alleles
- Animals
- Antigens, Protozoan
- Base Sequence
- Chromosome Mapping
- DNA Primers
- DNA, Protozoan
- Gene Amplification
- Genes, Protozoan
- Genetic Markers
- Humans
- Malaria, Falciparum
