Article
Conjugational recombination in Escherichia coli: genetic analysis of recombinant formation in Hfr x F- crosses.
Genetics - 1 Mar 1995
Lloyd R G, Buckman C
Abstract excerpt
The formation of recombinants during conjugation between Hfr and F- strains of Escherichia coli was investigated using unselected markers to monitor integration of Hfr DNA into the circular recipient chromosome. In crosses selecting a marker located approximately 500 kb from the Hfr origin, 60-70% of the recombinants appeared to inherit the Hfr DNA in a single segment, with the proximal exchange located > 300 kb...
Topics
- Chromosome Mapping
- Chromosomes, Bacterial
- Conjugation, Genetic
- DNA, Bacterial
- Escherichia coli
- Escherichia coli Proteins
- Exodeoxyribonuclease V
- Exodeoxyribonucleases
- Genetic Markers
- Genotype
- Models, Genetic
