Article
Cloning and sequence analysis of a cDNA for barley ferredoxin-dependent glutamate synthase and molecular analysis of photorespiratory mutants deficient in the enzyme.
Planta - 1 Jan 1993
Avila C, Márquez A J, Pajuelo P, Cannell M E, Wallsgrove R M, Forde B G
Abstract excerpt
The NH2-terminal sequences of ferredoxin-dependent glutamate synthase (Fd-GOGAT; EC 1.4.7.1) purified from barley (Hordeum vulgare L.) and Chlamydomonas reinhardtii (Dangeard), and of a barley peptide, were determined and the barley sequences were used to design oligonucleotide primers for the polymerase chain reaction. A specific 1.3-kilobase (kb) cDNA fragment specifying the NH2-terminal one-third of the mature...
Topics
- Amino Acid Oxidoreductases
- Amino Acid Sequence
- Animals
- Anthranilate Synthase
- Base Sequence
- Chlamydomonas reinhardtii
- Chromosome Mapping
- Cloning, Molecular
- DNA
- Hordeum
- Humans
