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Article

The functional role of glutamine-280 and threonine-282 in human alpha-galactosidase.

Biochimica et biophysica acta - 24 Apr 1995

Ishii S, Kase R, Sakuraba H, Suzuki Y

Abstract excerpt

Our previous study on chimeric mutants of alpha-galactosidase suggested that two peptide regions encoded by exons 1-2 and 6 of the enzyme gene contribute to substrate recognition (Ishii, S. et al. (1994) Biochim. Biophys. Acta 1204, 265-270). In this study, we constructed five single amino acid substitutions for functional analysis of the amino acid residues around glutamine-279, the mutation site detected in an...

Topics

Amino Acid Sequence
Base Sequence
Circular Dichroism
DNA Primers
Enzyme Stability
Humans
Hydrogen-Ion Concentration
In Vitro Techniques
Kinetics
Molecular Sequence Data
Mutagenesis, Site-Directed

Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

Mutation

Recombinant Fusion Proteins

Structure-Activity Relationship

alpha-Galactosidase

Source

Identifiers

PMID: 7727539
DOI: 10.1016/0925-4439(94)00082-2

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 29 Aug 2026. Prepared record publication-7727539-2026-08-29.1. Manifest publication-graph-cohort-2026-08-29.1.

Source receipt: PubMed via Helena Literature Corpus, retrieved 29 Aug 2026.

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