An exon 8-spliced out transcript of neurofibromatosis 2 gene is constitutively expressed in various human tissues.

Abstract excerpt

We succeeded in cloning the exon 8-spliced out transcript of neurofibromatosis 2 (NF2) gene in human glioma cell lines. We then investigated the expression of the spliced out form in various human tissues by PCR analysis followed by Southern blot hybridization and sequencing to evaluate whether it was generated by normal alternative splicing or by a splicing mutation. The analysis revealed the splicing out of...

Topics

• Base Sequence
• Cloning, Molecular
• Exons
• Gene Expression
• Genes, Neurofibromatosis 2
• Glioma
• Humans
• Male
• Molecular Sequence Data
• Mutation
• Polymerase Chain Reaction