Article
Immunodetection, expression strategy and complementation of turnip crinkle virus p28 and p88 replication components.
Virology - 20 Aug 1995
White K A, Skuzeski J M, Li W, Wei N, Morris T J
Abstract excerpt
The plus-sense RNA genome of turnip crinkle virus (TCV) encodes at its 5' end a 28-kDa protein of unspecified function. Readthrough suppression of the p28 stop codon allows for the production of an 88-kDa product which is required for genome replication. Immunological analysis of the expression of p28 and p88 demonstrated that: (i) the genome directs the synthesis of polypeptides of approximately 28 and 88 kDa,...
Topics
- Base Sequence
- Brassica
- Codon, Terminator
- DNA Primers
- DNA Replication
- Genetic Complementation Test
- Molecular Sequence Data
- Mutation
- Polymerase Chain Reaction
- Precipitin Tests
- RNA, Viral