Mutations in the processing site of the precursor of ribulose-1,5-bisphosphate carboxylase/oxygenase small subunit: effects on import, processing, assembly and stability.

Abstract excerpt

The small subunit (SSU) of Rubisco is synthesized in the cytosol in a precursor form. Upon import into the chloroplast, it is proteolytically processed at a Cys-Met bond to yield the mature form of the protein. To assess the importance of the Met residue for recognition and processing by the stromal peptidase, we substituted this residue with either Thr, Arg or Asp. The mutant precursor proteins were imported...

Topics

• Amino Acid Sequence
• Base Sequence
• Biological Transport
• Enzyme Precursors
• Enzyme Stability
• Molecular Sequence Data
• Mutagenesis, Site-Directed
• Mutation
• Protein Processing, Post-Translational
• Ribulose-Bisphosphate Carboxylase