Article
Role of PufX protein in photosynthetic growth of Rhodobacter sphaeroides. 1. PufX is required for efficient light-driven electron transfer and photophosphorylation under anaerobic conditions.
Biochemistry - 21 Nov 1995
Barz W P, Francia F, Venturoli G, Melandri B A, Verméglio A, Oesterhelt D
Abstract excerpt
The pufX gene is essential for photoheterotrophic growth of the purple bacterium Rhodobacter sphaeroides. In order to analyze the molecular function of the PufX membrane protein, we constructed a chromosomal pufX deletion mutant and phenotypically compared it to a pufX+ control strain and to two suppressor mutants which are able to grow photosynthetically in the absence of pufX. Using this genetic background, we...
Topics
- Adenosine Triphosphate
- Anaerobiosis
- Bacterial Proteins
- Base Sequence
- Cytochrome c Group
- Electrochemistry
- Electron Transport
- Gene Deletion
- Light
- Light-Harvesting Protein Complexes
