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Article

Characterization of PCR-ribotyping for Burkholderia (Pseudomonas) cepacia.

Journal of clinical microbiology - 1 Oct 1994

Dasen S E, LiPuma J J, Kostman J R, Stull T L

Abstract excerpt

Ribotyping, a method of genotyping bacterial isolates for epidemiologic study, uses rRNA as a probe to detect chromosomal restriction fragment length polymorphisms. Although ribotyping is accurate, its utility is limited by the labor and time necessary for Southern blot analysis. PCR-ribotyping uses PCR to amplify the 16S-23S intergenic spacer region of the bacterial rRNA operon. Length heterogeneity in the...

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Identifiers

PMID: 7529239
DOI: 10.1128/jcm.32.10.2422-2424.1994
PMCID: PMC264078

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