Article
Use of primers selective for vancomycin resistance genes to determine van genotype in enterococci and to study gene organization in VanA isolates.
Antimicrobial agents and chemotherapy - 1 Aug 1995
Miele A, Bandera M, Goldstein B P
Abstract excerpt
Vancomycin resistance in enterococci is an emerging therapeutic problem. Resistance is not always detected by standard microbiological methods. Oligonucleotide primers for PCR were designed to target amplification of defined regions of genes of the vanA cluster, as well as vanB and vanC1. These p...
Topics
- Anti-Bacterial Agents
- Base Sequence
- Blotting, Southern
- DNA Primers
- DNA, Bacterial
- Drug Resistance, Microbial
- Electrophoresis, Polyacrylamide Gel
- Enterococcus
- Genotype
- Molecular Sequence Data
- Nucleic Acid Hybridization
- Polymerase Chain Reaction
- Vancomycin