Article
Intracellular carbonic anhydrase of Chlamydomonas reinhardtii.
Plant physiology - 1 Oct 1995
Karlsson J, Hiltonen T, Husic H D, Ramazanov Z, Samuelsson G
Abstract excerpt
An intracellular carbonic anhydrase (CA; EC 4.2.1.1) was purified to homogeneity from a mutant strain of Chlamydomonas reinhardtii (CW 92) lacking a cell wall. Intact cells were washed to remove periplasmic CA and were lysed and fractionated into soluble and membrane fractions by sedimentation. All of the CA activity sedimented with the membrane fraction and was dissociated by treatment with a buffer containing...
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