Article
Development of a species-specific polymerase chain reaction assay for Gardnerella vaginalis.
Molecular and cellular probes - 1 Jun 1995
van Belkum A, Koeken A, Vandamme P, van Esbroeck M, Goossens H, Koopmans J, Kuijpers J, Falsen E, Quint W
Abstract excerpt
The nucleotide sequence of the region between the 16S and 23S rRNA genes of the facultative anaerobic bacterium Gardnerella vaginalis has been determined, together with the 5' proximal 500 nucleotides of the 23S rRNA gene. Regions suited for the development of specific, probe-confirmable polymerase chain reaction (PCR) assays were selected. PCR assays were evaluated with respect to sensitivity and specificity,...
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