Development of a species-specific polymerase chain reaction assay for Gardnerella vaginalis.

doi:10.1006/mcpr.1995.0024

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Article

Development of a species-specific polymerase chain reaction assay for Gardnerella vaginalis.

Molecular and cellular probes - 1 Jun 1995

van Belkum A, Koeken A, Vandamme P, van Esbroeck M, Goossens H, Koopmans J, Kuijpers J, Falsen E, Quint W

Abstract excerpt

The nucleotide sequence of the region between the 16S and 23S rRNA genes of the facultative anaerobic bacterium Gardnerella vaginalis has been determined, together with the 5' proximal 500 nucleotides of the 23S rRNA gene. Regions suited for the development of specific, probe-confirmable polymerase chain reaction (PCR) assays were selected. PCR assays were evaluated with respect to sensitivity and specificity,...

Topics

Base Sequence
DNA, Bacterial
DNA, Ribosomal
Fatty Acids
Female
Gardnerella vaginalis
Humans
Infant, Newborn
Molecular Sequence Data
Phenotype
Polymerase Chain Reaction

Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

Pregnancy

Pregnancy Complications, Infectious

RNA, Ribosomal

RNA, Ribosomal, 16S

RNA, Ribosomal, 23S

Species Specificity

Vaginosis, Bacterial

Source

Open in PubMed

Identifiers

PMID: 7477009
DOI: 10.1006/mcpr.1995.0024

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 29 Aug 2026. Prepared record publication-7477009-2026-08-29.1. Manifest publication-graph-cohort-2026-08-29.1.

Source receipt: PubMed via Helena Literature Corpus, retrieved 29 Aug 2026.

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