Benchmarking biofilm assays, surface models, and detachment methods in Pseudomonas aeruginosa for reproducible downstream molecular analysis.

Abstract excerpt

Pseudomonas aeruginosa is a major ESKAPE pathogen and a WHO priority pathogen, but biofilm studies remain difficult to compare because detection methods, test surfaces, strain selection, and biofilm recovery procedures vary widely across laboratories. This study evaluated three linked methodological questions: phenotypic biofilm detection, surface-associated expression of selected biofilm genes, and biofilm...

Topics

• Biofilms
• Pseudomonas aeruginosa
• Humans
• Bacteriological Techniques
• Pseudomonas Infections
• Bacterial Adhesion