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SARS-CoV-2 detection in wastewater using multiplex quantitative PCR

14 Jul 2021

Abstract excerpt

A multiplex reverse transcription quantitative PCR (RT-qPCR)-based method was designed for the simultaneous detection of different SARS-CoV-2 genes. In this study, we used three target genes encoding for the nucleocapsid 1 and 3 (N1, N3), and the spike (S) proteins, all commonly used in the detection of SARS-CoV-2 in human and environmental samples. The performance of the multiplex assay, compared to the single...

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PMID: 34298359
DOI: 10.1016/j.scitotenv.2021.148890

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