FACS-Proteomics strategy toward extracellular vesicles single-phenotype characterization in biological fluids: exploring the role of leukocyte-derived EVs in multiple sclerosis.

doi:10.1186/s12967-025-06558-4

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Article

FACS-Proteomics strategy toward extracellular vesicles single-phenotype characterization in biological fluids: exploring the role of leukocyte-derived EVs in multiple sclerosis.

Journal of translational medicine - 20 May 2025

Cufaro Maria Concetta, Lanuti Paola, De Bellis Domenico, Veschi Serena, Piro Anna, Fontana Antonella, Di Sebastiano Alice, Brocco Davide, Simeone Pasquale, Pilato Serena, Khorooshi Reza M H, Tomassini Valentina, Rispoli Marianna Gabriella, Federici Luca, Cicalini Ilaria, Pieragostino Damiana, Del Boccio Piero

Abstract excerpt

BACKGROUND: The isolation and proteomics characterization of extracellular vesicles (EVs) from body fluids is challenging due to their vast heterogeneity. We have recently demonstrated that Fluorescence-activated Cell Sorting (FACS) efficiently isolates the whole EV circulating compartment directly from untouched body fluids enabling a comprehensive EV proteomics analysis. RESULTS: Here, we characterized, for the...

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Topics

Humans
Extracellular Vesicles
Proteomics
Multiple Sclerosis

Methodology Data sources Editorial policy Privacy Delete account & data About Contact Helena Bioinformatics

Flow Cytometry

Phenotype

Leukocytes

Body Fluids

Female

Male

Adult

Biomarkers

Tears

Middle Aged

Source

Open in PubMed Open full text

Identifiers

PMID: 40394633
DOI: 10.1186/s12967-025-06558-4
PMCID: PMC12093873

Genes

NFE2L2
TGFB1

Provenance

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