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A Tear-Based Approach for Rapid Identification of Bacterial Pathogens in Corneal Ulcers Using Nanopore Sequencing

17 Apr 2025

Abstract excerpt

Purpose: This prospective observational study assesses the efficacy of using portable next-generation sequencing directly on tear samples to identify bacterial pathogens in corneal ulcers. Methods: Tear samples were collected from ulcerated and contralateral eyes using Schirmer strips. Corneal scrapings and cultures were performed as medically indicated. The 16S rRNA gene was amplified from tear samples using...

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Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

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PMID: 40244576
DOI: 10.1167/tvst.14.4.19

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