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Transcriptome analysis to identify genes related to programmed cell death resulted from manipulating of BnaFAH ortholog by CRISPR/Cas9 in Brassica napus.

Scientific reports - 2 Nov 2024

Zhou Zhou, Zhi Tiantian, Zou Jie, Chen Gang

Abstract excerpt

Fumarylacetoacetate hydrolase (FAH) catalyzes the final step of the tyrosine degradation pathway. In this study, we isolated and characterized two homologous BnaFAH genes in Brassica napus L. variant Westar, and then used CRISPR/Cas9-mediated targeted mutagenesis to generate a series of transgene-free mutant lines either with single or double-null bnafah alleles. Among these mutant lines, the aacc (bnafah)...

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Identifiers

PMID: 39488592
DOI: 10.1038/s41598-024-77877-7
PMCID: PMC11531537

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