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Article

Engineering quantitative stomatal trait variation and local adaptation potential by cis-regulatory editing.

Plant biotechnology journal - 1 Dec 2024

Karavolias Nicholas G, Patel-Tupper Dhruv, Gallegos Cruz Ana, Litvak Lillian, Lieberman Samantha E, Tjahjadi Michelle, Niyogi Krishna K, Cho Myeong-Je, Staskawicz Brian J

Abstract excerpt

Cis-regulatory element editing can generate quantitative trait variation that mitigates extreme phenotypes and harmful pleiotropy associated with coding sequence mutations. Here, we applied a multiplexed CRISPR/Cas9 approach, informed by bioinformatic datasets, to generate genotypic variation in the promoter of OsSTOMAGEN, a positive regulator of rice stomatal density. Engineered genotypic variation corresponded...

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Share to Adaptation, Physiological
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Methodology Data sources Editorial policy Privacy Delete account & data About Contact Helena Bioinformatics

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Share to Promoter Regions, Genetic

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Source

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Identifiers

PMID: 39425265
DOI: 10.1111/pbi.14464
PMCID: PMC11606412

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