Article
Structural insight into the Arabidopsis vacuolar anion channel ALMT9 shows clade specificity.
Cell reports - 24 Sept 2024
Qian Dandan, Chai Yaru, Li Weiping, Cui Bin, Lin Shaoquan, Wang Zhibin, Wang Chongyuan, Qu Le Qing, Gong Deshun
Abstract excerpt
The Arabidopsis thaliana aluminum-activated malate transporter 9 (AtALMT9) functions as a vacuolar chloride channel that regulates the stomatal aperture. Here, we present the cryoelectron microscopy (cryo-EM) structures of AtALMT9 in three distinct states. AtALMT9 forms a dimer, and the pore is lined with four positively charged rings. The apo-AtALMT9 state shows a putative endogenous citrate obstructing the...
Topics
- Arabidopsis
- Arabidopsis Proteins
- Vacuoles
- Organic Anion Transporters
- Cryoelectron Microscopy
- Mutation
- Models, Molecular
- Ion Channel Gating
- Chloride Channels