Article
Detection and differentiation of low virulence and virulent Orthoavulavirus javaense using a molecular beacon with RT-LAMP.
Scientific reports - 5 Aug 2024
Mears Megan C, Olivier Timothy L, Williams-Coplin Dawn, Espinoza Edna, Bakre Abhijeet
Abstract excerpt
Newcastle disease (ND), an economically important disease in poultry, is caused by virulent strains of the genetically diverse Orthoavulavirus javaense (OAVJ). Laboratories rely on quantitative real-time reverse transcription PCR (qRT-PCR) to detect OAVJ and differentiate between OAVJ pathotypes. This study demonstrates that a fusion cleavage site based molecular beacon with reverse transcription loop mediated...
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