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Article

High-sensitivity multiplex real-time RT-PCR for universal detection and virulence typing of Newcastle disease virus in poultry field samples.

Poultry science - 1 Jun 2026

Song Hye-Soon, Kim Hyeon-Su, Kim Ji-Ye, Kwon Yong-Kuk, Her Moon, Kim Hye-Ryoung

Abstract excerpt

Newcastle disease virus (NDV) remains a major global threat to poultry production despite widespread vaccination, driven by the emergence of genetically diverse and virulent Class II strains. We developed and validated a one-step multiplex real-time RT-PCR assay enabling simultaneous universal detection of all Class II NDV genotypes and differentiation between virulent (velogenic/mesogenic) and non-virulent...

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Identifiers

PMID: 41863979
DOI: 10.1016/j.psj.2026.106790
PMCID: PMC13018939

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