Using QUASR-PCR as a field-based genotyping assay for a tick acaricide resistance marker.

Abstract excerpt

A novel, turnkey, field-based workflow was developed and validated using Rhipicephalus microplus DNA as a template to detect the presence of the voltage-gated sodium channel kdr mutation. The field-based compatible workflow comprises manual sample homogenization for DNA extraction, PCR amplification of the targets in a closed tube, and end-point detection of the PCR products. An R. microplus species-specific...

Topics

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• Acaricides
• Drug Resistance
• Genotype
• Genotyping Techniques
• Mutation
• Polymerase Chain Reaction