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Development of two multiplex PCR assays for rapid detection of eleven Gram-negative bacteria in children with septicemia

5 Jun 2024

Abstract excerpt

AIM: This study aimed to develop a multiplex PCR assay for simultaneous detection of major Gram-negative etiologies of septicemia and evaluate its performance. METHODS: Multiplex PCR (mPCR) assays were developed targeting 11 bacterial strains. Species-specific primers were confirmed using known clinical isolates and standard strains. Gradient PCR was performed on each primer against its target bacterial gene to...

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Share to Bacterial Identification and Susceptibility Testing
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PMID: 38840209
DOI: 10.1186/s41182-024-00606-3

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