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Evaluation of 16S rRNA broad range PCR assay for microbial detection in serum specimens in sepsis patients

12 Feb 2020

Abstract excerpt

BACKGROUND: Early and accurate laboratory diagnosis and appropriate management of infection improves the survival rate in sepsis. In this study we evaluated broad range 16S rRNA and 16 S-23 S intergenic spacer region (ISR) PCR assays followed by nucleotide sequencing directly from patients' serum and automated blood culture for laboratory diagnosis in admitted sepsis patients. METHODS: A broad range 16S rRNA PCR...

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PMID: 32061569
DOI: 10.1016/j.jiph.2020.01.007

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