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KP177R-based visual assay integrating RPA and CRISPR/Cas12a for the detection of African swine fever virus

9 Apr 2024

Abstract excerpt

Introduction Early detection of the virus in the environment or in infected pigs is a critical step to stop African swine fever virus (ASFV) transmission. The p22 protein encoded by ASFV KP177R gene has been shown to have no effect on viral replication and virulence and can serve as a molecular marker for distinguishing field virus strains from future candidate KP177R deletion vaccine strains. Methods This study...

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PMID: 38655256
DOI: 10.3389/fimmu.2024.1358960

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