Article
New Allele-Specific Oligonucleotide (ASO) amplifications for Toxoplasma gondii rop18 allele typing: Analysis of 86 human congenital infections in Brazil.
Acta tropica - 1 Nov 2023
Dos Santos Emilly Henrique, Barreira Gabriel Acca, Yamamoto Lidia, Rocha Mussya Cisotto, Rodrigues Karen Alessandra, Cruz Maria Carolina Pires, Kanunfre Kelly Aparecida, Okay Thelma Suely
Abstract excerpt
This study aimed to detect and differentiate Toxoplasma gondii by the allele typing of its polymorphic rop18 gene. For this purpose, a novel genotyping system using allele-specific oligonucleotides (ASOs) was designed, consisting of three ASO pairs. The first and third pairs specifically amplify rop18 allele I and allele III, while the second pair amplify both allele I and II. Genomic DNA from 86 congenital...
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