Article
Discrimination of SARS-CoV-2 omicron variant and its lineages by rapid detection of immune-escape mutations in spike protein RBD using asymmetric PCR-based melting curve analysis.
Virology journal - 25 Aug 2023
Kong Xiaomu, Gao Peng, Jiang Yongwei, Lu Lixia, Zhao Meimei, Liu Yi, Deng Guoxiong, Zhu Haoyan, Cao Yongtong, Ma Liang
Abstract excerpt
BACKGROUND: The SARS-CoV-2 Omicron strain has multiple immune-escape mutations in the spike protein receptor-binding domain (RBD). Rapid detection of these mutations to identify Omicron and its lineages is essential for guiding public health strategies and patient treatments. We developed a two-tube, four-color assay employing asymmetric polymerase chain reaction (PCR)-based melting curve analysis to detect...
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