Article
Development of a Real-Time PCR Assay for Detection of Macrolide Resistance Mutations in Mycoplasma genitalium and Its Application for Epidemiological Surveillance in Russia.
Microbial drug resistance (Larchmont, N.Y.) - 1 Mar 2023
Edelstein Inna Alexandrovna, Romanov Andrey Vyacheslavovich, Kozlov Roman Sergeevich
Abstract excerpt
A real-time PCR assay based on probe fluorescence quenching by a primer was developed and validated for detection of macrolide resistance (MR)-associated mutations in the 23S rRNA gene of Mycoplasma genitalium. The assay involves identification of any nucleotide substitutions at positions 2058, 2059, and 2611 of 23S rRNA (Escherichia coli numbering) by the probe-based melting curve analysis immediately after...
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