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A multiplexed, paired-pooled droplet digital PCR assay for detection of SARS-CoV-2 in saliva

22 Feb 2023

Abstract excerpt

In response to the SARS-CoV-2 pandemic, we developed a multiplexed, paired-pool droplet digital PCR (MP4) screening assay. Key features of our assay are the use of minimally processed saliva, 8-sample paired pools, and reverse-transcription droplet digital PCR (RT-ddPCR) targeting the SARS-CoV-2 nucleocapsid gene. The limit of detection was determined to be 2 and 12 copies per µl for individual and pooled...

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PMID: 36813822
DOI: 10.1038/s41598-023-29858-5

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