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Article

RT-qPCR genotyping assays for differentiating Rift Valley fever phlebovirus strains.

Journal of virological methods - 1 May 2023

Balaraman Velmurugan, Gaudreault Natasha N, Trujillo Jessie D, Indran Sabarish V, Wilson William C, Richt Juergen A

Abstract excerpt

Rift Valley fever phlebovirus (RVFV) is an emerging, mosquito-borne, zoonotic pathogen. Real time RT-qPCR genotyping (GT) assays were developed to differentiate between two RVFV wild-type strains (128B-15 and SA01-1322) and a vaccine strain (MP-12). The GT assay uses a one-step RT-qPCR mix, with two different RVFV strain-specific primers (either forward or reverse) with long or short G/C tags and a common primer...

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Identifiers

PMID: 36801236
DOI: 10.1016/j.jviromet.2023.114693
PMCID: PMC10040438

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