Article
Comparative analysis of A1 and A2 allele detection efficiency for bovine CSN2 gene by AS-PCR methods.
Acta biochimica Polonica - 11 Feb 2023
Kulibaba Roman, Sakhatskyi Mykola, Liashenko Yuriy
Abstract excerpt
This study was aimed at conducting a comparative analysis of the efficiency in genotyping cattle by beta-casein locus using the allele-specific PCR methods. The results of the study have demonstrated the necessity to optimize the protocol for the use of AS-PCR to detect alleles A1 and A2. It was found that the use of non-optimized PCR protocols led to the genotyping errors, manifested regarding beta-casein locus...
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