Article
Technical note: detection of the C allele of beta-casein (CSN2) in Czech Dairy goat breeds using LightCycler analysis.
Journal of dairy science - 1 Oct 2008
Sztankóová Z, Kysel'ová J, Kott T, Kottová E
Abstract excerpt
A protocol was developed for rapid genotyping of A and C variants at the CSN2 locus in goat species (White Shorthaired and Brown Shorthaired goat) by PCR and LightCycler analysis. The LightCycler technique combines rapid and efficient in vitro amplification of DNA in glass capillaries, with melting curve analysis based on fluorescence resonance energy transfer, for the sensitive detection of point mutation....
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