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Shotgun metagenomic sequencing in culture negative microbial keratitis

8 Jan 2023

Abstract excerpt

Purpose To evaluate the microbiota of culture negative Corneal Impression Membrane (CIM) microbial keratitis samples with the use of shotgun metagenomics analysis. Methods DNA of microbial keratitis samples were collected with CIM and extracted using the MasterPure™ Complete DNA and RNA Purification Kit (Epicentre). DNA was fragmented by sonication into fragments of 300 to 400 base pairs (bp) using Bioruptor®...

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PMID: 36617769
DOI: 10.1177/11206721221149077

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