Article
[Selection of mutants of Clostridium acetobutylicum defective in production of acetone].
Annales de l'Institut Pasteur. Microbiology - 1 Jan 2000
Janati-Idrissi R, Junelles A M, el Kanouni A, Petitdemange H, Gay R
Abstract excerpt
The butyrate analogue 2-bromobutyrate is toxic toward Clostridium acetobutylicum. After mutation using nitrosoguanidine, mutants resistant to the suicide co-substrate 2-bromobutyrate were selected. Such mutants no longer produced acetone, whereas the synthesis of butanol and ethanol was unaffected. Enzymatic analysis of a 2-bromobutyrate-resistant mutant showed a low level of coenzyme A transferase and of...
Topics
- Acetone
- Bromides
- Butyrates
- Clostridium
- Drug Resistance, Microbial
- Mutation