Article
Mass Cytometry Assessment of Cell Phenotypes and Signaling States in Human Whole Blood.
Methods in molecular biology (Clifton, N.J.) - 1 Jan 2022
Sigal Natalia, Maecker Holden T
Abstract excerpt
Phosphoflow is a powerful tool that allows researchers to measure distinct signaling responses to various stimuli in multiple subpopulations of cells. Extension of this technique to mass cytometry (cytometry by time-of-flight or CyTOF) allows many more cell phenotypes and signaling nodes to be interrogated in parallel. The use of fresh whole blood is ideal for capturing the in vivo signaling state of all...
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