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CRISPR-Cas13a Based Visual Detection Assays for Feline Calicivirus Circulating in Southwest China

11 Jul 2022

Abstract excerpt

Feline calicivirus (FCV) is a well-known causative pathogen for upper respiratory infection in cats. Its high genetic variability challenges existing molecular diagnostic methods in clinical settings. Thus, we developed two sensitive and visual assays for FCV nucleic acid detection based on RPA reaction and CRISPR-Cas13a trans-cleavage activity. Recombinant plasmid DNA, crRNAs, and RPA primers were designed and...

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PMID: 35898546
DOI: 10.3389/fvets.2022.913780

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