Article
[Phenotyping of peripheral lymphocytes with monoclonal antibodies in newborn infants and adults].
La Pediatria medica e chirurgica : Medical and surgical pediatrics - 1 Jan 2000
Poccardi G, Pilotti G, Magrizos C, Sala U, Castelli G, Pennacino G, Remogna M
Abstract excerpt
The authors investigated and compared lymphocyte subsets natural killer in 33 newborns and in 33 healthy adults. From 5 ml of heparinized venous blood were separated mononuclear cells on density-gradient Ficoll-Hypaque. The vitality was determined with immunofluorescent method (orange acridine and ethiobromide). It was positive if resulted at least 95%. Lymphocyte phenotype was also examined after incubation with...
Topics
- Adult
- Aging
- Antibodies, Monoclonal
- B-Lymphocytes
- Female
- Humans
- Infant, Newborn
- Lymphocytes
- Male
- Phenotype
- T-Lymphocytes