Article
dnaJ: a New Approach to Identify Species within the Genus Enterobacter.
Microbiology spectrum - 22 Dec 2021
Hernandez-Alonso Enrique, Barreault Simon, Augusto Luis A, Jatteau Pierre, Villet Millie, Tissieres Pierre, Doucet-Populaire Florence, Bourgeois-Nicolaos Nadege
Abstract excerpt
The taxonomy of the genus Enterobacter can be confusing and has been considerably revised in recent years. We propose a PCR and amplicon sequencing technique based on a partial sequence of the dnaJ gene for species assignment consistent with DNA-DNA digital hybridization (dDDH) and pairwise average nucleotide identity (ANI). We performed a validation of the method by comparing the type strains of each species,...
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