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Article

A rapid and low-cost protocol for the detection of B.1.1.7 lineage of SARS-CoV-2 by using SYBR Green-based RT-qPCR.

Molecular biology reports - 1 Nov 2021

Abdel Sater Fadi, Younes Mahmoud, Nassar Hassan, Nguewa Paul, Hamze Kassem

Abstract excerpt

BACKGROUND: The new SARS-CoV-2 variant VOC (202012/01), identified recently in the United Kingdom (UK), exhibits a higher transmissibility rate compared to other variants, and a reproductive number 0.4 higher. In the UK, scientists were able to identify the increase of this new variant through the rise of false negative results for the spike (S) target using a three-target RT-PCR assay (TaqPath kit). METHODS: To...

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PMID: 34613565
DOI: 10.1007/s11033-021-06717-y
PMCID: PMC8493944

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