Loading publication...

Noodle always counts identifier-free feature events for service operation. With your permission, Google Analytics can also estimate audience and engagement. Search text, publication identifiers, cookies, IP addresses and browser fingerprints are never included in Noodle's first-party event counters.

Privacy details

Topics Community

Article

Multiple Occurrences of a 168-Nucleotide Deletion in SARS-CoV-2 ORF8, Unnoticed by Standard Amplicon Sequencing and Variant Calling Pipelines.

Viruses - 18 Sept 2021

Brandt David, Simunovic Marina, Busche Tobias, Haak Markus, Belmann Peter, Jünemann Sebastian, Schulz Tizian, Klages Levin Joe, Vinke Svenja, Beckstette Michael, Pohl Ehmke, Scherer Christiane, Sczyrba Alexander, Kalinowski Jörn

Abstract excerpt

Genomic surveillance of the SARS-CoV-2 pandemic is crucial and mainly achieved by amplicon sequencing protocols. Overlapping tiled-amplicons are generated to establish contiguous SARS-CoV-2 genome sequences, which enable the precise resolution of infection chains and outbreaks. We investigated a SARS-CoV-2 outbreak in a local hospital and used nanopore sequencing with a modified ARTIC protocol employing 1200 bp...

Topics

COVID-19
Genes, Viral
Genetic Variation
Humans
Nanopore Sequencing
Open Reading Frames
SARS-CoV-2
Sequence Analysis, RNA
Sequence Deletion
Whole Genome Sequencing

Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

Source

Open in PubMed Open full text

Identifiers

PMID: 34578452
DOI: 10.3390/v13091870
PMCID: PMC8518987

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 31 Aug 2026.

Community interpretation

Discuss the evidence

Ask a question, add context or challenge an interpretation. Community activity is separate from scientific evidence strength.

0

discussions

0

replies

Citations encouraged

Claims remain attributable.

No discussion yet

Be the first to add a careful, cited interpretation.

Related research

Semantic proximity does not establish scientific evidence.

Research trail→

Click a neighbor to travelStep 1 · 12 closest

Multiple Occurrences of a 168-Nucleotide Deletion in SARS-CoV-2 ORF8, Unnoticed by Standard Amplicon Sequencing and Variant Calling Pipelines.PMID 34578452Read ↗

Recommendations for Uniform Variant Calling of SARS-CoV-2 Genome Sequence across Bioinformatic WorkflowsSemantic proximity Read ↗

Analytical validity of nanopore sequencing for rapid SARS-CoV-2 genome analysisSemantic proximity Read ↗

Analytical validity of nanopore sequencing for rapid SARS-CoV-2 genome analysisSemantic proximity Read ↗

Detection of SARS-CoV-2 Δ426 ORF8 Deletion Mutant Cluster in NGS ScreeningSemantic proximity Read ↗

A deletion in SARS‐CoV‐2 ORF7 identified in COVID‐19 outbreak in UruguaySemantic proximity Read ↗

Rapid Genomic Characterization of SARS-CoV-2 Viruses From Clinical Specimens Using Nanopore SequencingSemantic proximity Read ↗

Performance of amplicon and capture based next-generation sequencing approaches for the epidemiological surveillance of Omicron SARS-CoV-2 and other variants of concern.Semantic proximity Read ↗

Performance of amplicon and capture based next-generation sequencing approaches for the epidemiological surveillance of Omicron SARS-CoV-2 and other variants of concernSemantic proximity Read ↗

Simplified point-of-care full SARS-CoV-2 genome sequencing using nanopore technologySemantic proximity Read ↗

Simplified Point-of-Care Full SARS-CoV-2 Genome Sequencing Using Nanopore TechnologySemantic proximity Read ↗

Rapid genomic characterization of SARS-CoV-2 viruses from clinical specimens using nanopore sequencing.Semantic proximity Read ↗

Nanopore sequencing of SARS-CoV-2: Comparison of short and long PCR-tiling amplicon protocolsSemantic proximity Read ↗

⌁Select a neighboring publication to make it the new centre.