Article
Bovine leukemia virus genotype surveillance in cattle at a slaughterhouse in Aichi Prefecture, Japan, in 2019 using polymerase chain reaction combined with restriction fragment length polymorphism.
The Journal of veterinary medical science - 16 Nov 2021
Sahashi Yuma, Oshima Masaaki, Yamagishi Junjiro, Muramatsu Chieko, Shimizu Kaori, Inoshima Yasuo
Abstract excerpt
Polymerase chain reaction (PCR) combined with restriction fragment length polymorphism (RFLP) is commonly used for genotyping bovine leukemia virus (BLV) in slaughterhouses. However, unclassified BLV genotypes have been sporadically reported. To assess the current status of BLV genetic characterization in cattle, PCR-RFLP was performed on blood samples of 170 cattle (84 Japanese Black, 60 Japanese Black x...
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