Article
Simultaneous genotyping of snails and infecting trematode parasites using high-throughput amplicon sequencing.
Molecular ecology resources - 1 Feb 2022
Hammoud Cyril, Mulero Stephen, Van Bocxlaer Bert, Boissier Jérôme, Verschuren Dirk, Albrecht Christian, Huyse Tine
Abstract excerpt
Several methodological issues currently hamper the study of entire trematode communities within populations of their intermediate snail hosts. Here we develop a new workflow using high-throughput amplicon sequencing to simultaneously genotype snail hosts and their infecting trematode parasites. We designed primers to amplify four snail and five trematode markers in a single multiplex PCR. While also applicable to...
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