Article
Discrepant Diagnostic Results of Nested Polymerase Chain Reaction-based Genotyping in a Patient with Hepatitis C Virus and Human Immunodeficiency Virus Coinfection.
Internal medicine (Tokyo, Japan) - 15 Oct 2021
Minemura Masami, Tajiri Kazuto, Hayashi Yuka, Takahashi Naoki, Watanabe Kasumi, Hanaoka Tatsuyuki, Araki Yasuhiro, Takahashi Kosuke, Takahara Terumi, Kojima Shigeyuki, Yasuda Ichiro
Abstract excerpt
Accurate genotyping is important to improve the treatment of hepatitis C virus (HCV) infection. We herein report a 44-year-old Japanese man with hemophilia A and coinfection of HCV and human immunodeficiency virus (HIV) who was diagnosed with HCV genotype 4 by direct sequencing. Two genotyping tests based on the nested polymerase chain reaction method that we used misdiagnosed his genotype as 2b and 1b. Although...
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