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Article

Effect of Long-Term 3D Spheroid Culture on WJ-MSC.

Cells - 24 Mar 2021

Kaminska Agnieszka, Wedzinska Aleksandra, Kot Marta, Sarnowska Anna

Abstract excerpt

The aim of our work was to develop a protocol enabling a derivation of mesenchymal stem/stromal cell (MSC) subpopulation with increased expression of pluripotent and neural genes. For this purpose we used a 3D spheroid culture system optimal for neural stem cells propagation. Although 2D culture conditions are typical and characteristic for MSC, under special treatment these cells can be cultured for a short time...

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Identifiers

PMID: 33804895
DOI: 10.3390/cells10040719
PMCID: PMC8063822

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